diff --git a/dom/bindings/mozwebidlcodegen/test/python.ini b/dom/bindings/mozwebidlcodegen/test/python.ini
index 0bfb2e5580a3..3b86feafb3d3 100644
--- a/dom/bindings/mozwebidlcodegen/test/python.ini
+++ b/dom/bindings/mozwebidlcodegen/test/python.ini
@@ -1,5 +1,4 @@
 [DEFAULT]
-skip-if = python == 2
 subsuite = mozbuild
 
 [test_mozwebidlcodegen.py]
diff --git a/python/mozbuild/mozbuild/test/python.ini b/python/mozbuild/mozbuild/test/python.ini
index 25d97cfe83f5..f7f6d2f96329 100644
--- a/python/mozbuild/mozbuild/test/python.ini
+++ b/python/mozbuild/mozbuild/test/python.ini
@@ -10,7 +10,6 @@ subsuite = mozbuild
 [backend/test_recursivemake.py]
 [backend/test_build.py]
 [backend/test_database.py]
-skip-if = python == 2
 [backend/test_configenvironment.py]
 [backend/test_gn_processor.py]
 [backend/test_partialconfigenvironment.py]
@@ -18,23 +17,17 @@ skip-if = python == 2
 [backend/test_visualstudio.py]
 [code_analysis/test_mach_commands.py]
 [codecoverage/test_lcov_rewrite.py]
-skip-if = python == 2
 [compilation/test_warnings.py]
 [configure/lint.py]
-skip-if = python == 2
 [configure/test_checks_configure.py]
 [configure/test_compile_checks.py]
 [configure/test_configure.py]
 [configure/test_lint.py]
-skip-if = python == 2
 [configure/test_moz_configure.py]
-skip-if = python == 2
 [configure/test_options.py]
 [configure/test_toolchain_configure.py]
-skip-if = python == 2
 [configure/test_toolchain_helpers.py]
 [configure/test_toolkit_moz_configure.py]
-skip-if = python == 2
 [configure/test_util.py]
 [controller/test_ccachestats.py]
 [controller/test_clobber.py]
@@ -59,11 +52,8 @@ skip-if = python == 2
 [test_preprocessor.py]
 [test_pythonutil.py]
 [test_rewrite_mozbuild.py]
-skip-if = os == "win" # normalizing pathnames doesn't work here yet
 [test_telemetry.py]
-skip-if = python == 2 && os == "mac"
 [test_telemetry_settings.py]
-skip-if = python == 2
 [test_util.py]
 [test_util_fileavoidwrite.py]
 [test_vendor.py]
diff --git a/python/mozbuild/mozpack/test/python.ini b/python/mozbuild/mozpack/test/python.ini
index e314edfd9393..281ce65c5000 100644
--- a/python/mozbuild/mozpack/test/python.ini
+++ b/python/mozbuild/mozpack/test/python.ini
@@ -7,7 +7,6 @@ subsuite = mozbuild
 [test_copier.py]
 [test_errors.py]
 [test_files.py]
-skip-if = python == 2 && os == "mac"
 [test_manifests.py]
 [test_mozjar.py]
 [test_packager.py]
@@ -16,4 +15,3 @@ skip-if = python == 2 && os == "mac"
 [test_packager_unpack.py]
 [test_path.py]
 [test_unify.py]
-skip-if = python == 2
diff --git a/python/mozlint/test/python.ini b/python/mozlint/test/python.ini
index 1de09c09657c..5c2c11d73ff1 100644
--- a/python/mozlint/test/python.ini
+++ b/python/mozlint/test/python.ini
@@ -1,6 +1,5 @@
 [DEFAULT]
 subsuite = mozlint
-skip-if = python == 2
 
 [test_cli.py]
 [test_editor.py]
diff --git a/taskcluster/test/python.ini b/taskcluster/test/python.ini
index c33cf6ac3a5e..637f250af27e 100644
--- a/taskcluster/test/python.ini
+++ b/taskcluster/test/python.ini
@@ -1,6 +1,5 @@
 [DEFAULT]
 subsuite = ci
-skip-if = python == 2
 
 [test_autoland.py]
 [test_autoland_backstop.py]
diff --git a/testing/mozbase/mozleak/tests/manifest.ini b/testing/mozbase/mozleak/tests/manifest.ini
index 0d9295037bdc..f15df4d06cd9 100644
--- a/testing/mozbase/mozleak/tests/manifest.ini
+++ b/testing/mozbase/mozleak/tests/manifest.ini
@@ -1,5 +1,4 @@
 [DEFAULT]
 subsuite = mozbase
-skip-if = os != "linux"
 
 [test_lsan.py]
diff --git a/testing/mozbase/moztest/tests/manifest.ini b/testing/mozbase/moztest/tests/manifest.ini
index b69b0ba4e6a6..292bb99adb0c 100644
--- a/testing/mozbase/moztest/tests/manifest.ini
+++ b/testing/mozbase/moztest/tests/manifest.ini
@@ -1,5 +1,6 @@
 [DEFAULT]
 subsuite = mozbase
+
 [test.py]
 [test_resolve.py]
-skip-if = python == 2
\ No newline at end of file
+
diff --git a/toolkit/crashreporter/tools/python.ini b/toolkit/crashreporter/tools/python.ini
index bee105d3e4ec..d715bfe094fa 100644
--- a/toolkit/crashreporter/tools/python.ini
+++ b/toolkit/crashreporter/tools/python.ini
@@ -1,4 +1,3 @@
 [DEFAULT]
-skip-if = python == 2
 
 [unit-symbolstore.py]
diff --git a/tools/fuzzing/smoke/python.ini b/tools/fuzzing/smoke/python.ini
index 33049f58a3d2..6701224f36ab 100644
--- a/tools/fuzzing/smoke/python.ini
+++ b/tools/fuzzing/smoke/python.ini
@@ -1,6 +1,5 @@
 [DEFAULT]
 subsuite = fuzzing
-skip-if = python == 2
 
 [test_grizzly.py]
 requirements = tools/fuzzing/smoke/grizzly_requirements.txt
diff --git a/tools/lint/test/python.ini b/tools/lint/test/python.ini
index 81790f7178a3..c478033738a9 100644
--- a/tools/lint/test/python.ini
+++ b/tools/lint/test/python.ini
@@ -1,15 +1,12 @@
 [DEFAULT]
 subsuite = mozlint
-skip-if = python == 2
 
 [test_black.py]
 requirements = tools/lint/python/black_requirements.txt
-skip-if = os == "mac"  # pip unable to find black
 [test_eslint.py]
 skip-if = os == "win"  # busts the tree for subsequent tasks on the same worker (bug 1708591)
 [test_flake8.py]
 requirements = tools/lint/python/flake8_requirements.txt
-skip-if = os == "mac"  # pip unable to find 'flake8==3.5.0'
 [test_fluent_lint.py]
 [test_file_perm.py]
 skip-if = os == "win"
@@ -19,9 +16,7 @@ skip-if = os == "win"
 [test_shellcheck.py]
 [test_rst.py]
 requirements = tools/lint/rst/requirements.txt
-skip-if = os == "mac" # pip unable to install
 [test_codespell.py]
-skip-if = os == "win" || os == "mac"  # codespell installed on Linux
 [test_yaml.py]
 [test_clippy.py]
 [test_rustfmt.py]
@@ -30,7 +25,5 @@ skip-if = os == "win" || os == "mac"  # codespell installed on Linux
 [test_perfdocs_helpers.py]
 [test_perfdocs_generation.py]
 [test_pylint.py]
-skip-if = os == "win" || os == "mac"  # only installed on linux
 requirements = tools/lint/python/pylint_requirements.txt
 [test_trojan_source.py]
-skip-if = os == "win"  # Python, Windows and UTF...
diff --git a/xpcom/ds/test/python.ini b/xpcom/ds/test/python.ini
index 0d2c6fd49b7b..680185c496d5 100644
--- a/xpcom/ds/test/python.ini
+++ b/xpcom/ds/test/python.ini
@@ -2,4 +2,3 @@
 subsuite = xpcom
 
 [test_dafsa.py]
-skip-if = python == 2
diff --git a/xpcom/idl-parser/xpidl/python.ini b/xpcom/idl-parser/xpidl/python.ini
index 5bb57b2300fd..96dbc4a9f1ff 100644
--- a/xpcom/idl-parser/xpidl/python.ini
+++ b/xpcom/idl-parser/xpidl/python.ini
@@ -1,5 +1,4 @@
 [DEFAULT]
-skip-if = python == 2
 subsuite = xpcom
 
 [runtests.py]