diff --git a/dom/webidl/Node.webidl b/dom/webidl/Node.webidl
index 9b9063fe2b6b..cc367adc093b 100644
--- a/dom/webidl/Node.webidl
+++ b/dom/webidl/Node.webidl
@@ -36,7 +36,7 @@ interface Node : EventTarget {
 
   [Pure]
   readonly attribute Document? ownerDocument;
-  [Pure]
+  [Pure, Pref="dom.node.rootNode.enabled"]
   readonly attribute Node rootNode;
   [Pure]
   readonly attribute Node? parentNode;
diff --git a/modules/libpref/init/all.js b/modules/libpref/init/all.js
index 676923f60444..1e74fef21f53 100644
--- a/modules/libpref/init/all.js
+++ b/modules/libpref/init/all.js
@@ -5335,3 +5335,10 @@ pref("dom.mozBrowserFramesEnabled", false);
 
 // Is support for 'color-adjust' CSS property enabled?
 pref("layout.css.color-adjust.enabled", true);
+
+// Disable Node.rootNode in release builds.
+#ifdef RELEASE_BUILD
+pref("dom.node.rootNode.enabled", false);
+#else
+pref("dom.node.rootNode.enabled", true);
+#endif
diff --git a/testing/web-platform/meta/dom/interfaces.html.ini b/testing/web-platform/meta/dom/interfaces.html.ini
index 5feed3166b6f..6cfa804e415c 100644
--- a/testing/web-platform/meta/dom/interfaces.html.ini
+++ b/testing/web-platform/meta/dom/interfaces.html.ini
@@ -1,5 +1,6 @@
 [interfaces.html]
   type: testharness
+  prefs: [dom.node.rootNode.enabled:true]
   [MutationObserver interface: operation observe(Node,MutationObserverInit)]
     expected: FAIL
 
diff --git a/testing/web-platform/meta/dom/nodes/rootNode.html.ini b/testing/web-platform/meta/dom/nodes/rootNode.html.ini
new file mode 100644
index 000000000000..50767cbf62d2
--- /dev/null
+++ b/testing/web-platform/meta/dom/nodes/rootNode.html.ini
@@ -0,0 +1,3 @@
+[rootNode.html]
+  type: testharness
+  prefs: [dom.node.rootNode.enabled:true]