diff --git a/browser/tools/mozscreenshots/mozscreenshots/extension/configurations/PermissionPrompts.jsm b/browser/tools/mozscreenshots/mozscreenshots/extension/configurations/PermissionPrompts.jsm
index afded5d854b3..b8a32f5a596a 100644
--- a/browser/tools/mozscreenshots/mozscreenshots/extension/configurations/PermissionPrompts.jsm
+++ b/browser/tools/mozscreenshots/mozscreenshots/extension/configurations/PermissionPrompts.jsm
@@ -20,8 +20,6 @@ this.PermissionPrompts = {
   init(libDir) {
     Services.prefs.setBoolPref("browser.storageManager.enabled", true);
     Services.prefs.setBoolPref("media.navigator.permission.fake", true);
-    Services.prefs.setCharPref("media.getusermedia.screensharing.allowed_domains",
-                               "test1.example.com");
     Services.prefs.setBoolPref("extensions.install.requireBuiltInCerts", false);
     Services.prefs.setBoolPref("signon.rememberSignons", true);
   },
diff --git a/dom/media/tests/mochitest/head.js b/dom/media/tests/mochitest/head.js
index 1841b71674d3..a06caab37e50 100644
--- a/dom/media/tests/mochitest/head.js
+++ b/dom/media/tests/mochitest/head.js
@@ -322,7 +322,6 @@ function setupEnvironment() {
       ['media.navigator.permission.disabled', true],
       ['media.navigator.streams.fake', FAKE_ENABLED],
       ['media.getusermedia.screensharing.enabled', true],
-      ['media.getusermedia.screensharing.allowed_domains', "mochi.test"],
       ['media.getusermedia.audiocapture.enabled', true],
       ['media.recorder.audio_node.enabled', true]
     ]
diff --git a/dom/media/tests/mochitest/test_getUserMedia_basicScreenshare.html b/dom/media/tests/mochitest/test_getUserMedia_basicScreenshare.html
index 76f3521a0511..227a63aae5e8 100644
--- a/dom/media/tests/mochitest/test_getUserMedia_basicScreenshare.html
+++ b/dom/media/tests/mochitest/test_getUserMedia_basicScreenshare.html
@@ -73,10 +73,7 @@
         return;
     }
 
-    // Screensharing must work even without "mochi.test," in allowed_domains
     await pushPrefs(
-      ["media.getusermedia.screensharing.allowed_domains",
-        "mozilla.github.io,*.bugzilla.mozilla.org"],
       ["full-screen-api.enabled", true],
       ["full-screen-api.unprefix.enabled", true],
       ["full-screen-api.allow-trusted-requests-only", false],
diff --git a/modules/libpref/init/all.js b/modules/libpref/init/all.js
index 3d397a6c3b3c..dff9935b58dd 100644
--- a/modules/libpref/init/all.js
+++ b/modules/libpref/init/all.js
@@ -560,13 +560,6 @@ pref("dom.webaudio.enabled", true);
 pref("media.getusermedia.screensharing.enabled", true);
 #endif
 
-#ifdef RELEASE_OR_BETA
-pref("media.getusermedia.screensharing.allowed_domains", "webex.com,*.webex.com,ciscospark.com,*.ciscospark.com,projectsquared.com,*.projectsquared.com,*.room.co,room.co,beta.talky.io,talky.io,*.clearslide.com,appear.in,*.appear.in,tokbox.com,*.tokbox.com,*.sso.francetelecom.fr,*.si.francetelecom.fr,*.sso.infra.ftgroup,*.multimedia-conference.orange-business.com,*.espacecollaboration.orange-business.com,free.gotomeeting.com,g2m.me,*.g2m.me,*.mypurecloud.com,*.mypurecloud.com.au,spreed.me,*.spreed.me,*.spreed.com,air.mozilla.org,*.circuit.com,*.yourcircuit.com,circuit.siemens.com,yourcircuit.siemens.com,circuitsandbox.net,*.unify.com,tandi.circuitsandbox.net,*.ericsson.net,*.cct.ericsson.net,*.opentok.com,*.conf.meetecho.com,meet.jit.si,*.meet.jit.si,web.stage.speakeasyapp.net,web.speakeasyapp.net,*.hipchat.me,*.beta-wspbx.com,*.wspbx.com,*.unifiedcloudit.com,*.smartboxuc.com,*.smartbox-uc.com,*.panterranetworks.com,pexipdemo.com,*.pexipdemo.com,pex.me,*.pex.me,*.rd.pexip.com,1click.io,*.1click.io,*.fuze.com,*.fuzemeeting.com,*.thinkingphones.com,gotomeeting.com,*.gotomeeting.com,gotowebinar.com,*.gotowebinar.com,gototraining.com,*.gototraining.com,citrix.com,*.citrix.com,expertcity.com,*.expertcity.com,citrixonline.com,*.citrixonline.com,g2m.me,*.g2m.me,gotomeet.me,*.gotomeet.me,gotomeet.at,*.gotomeet.at,miriadaxdes.miriadax.net,certificacion.miriadax.net,miriadax.net,*.wire.com,sylaps.com,*.sylaps.com,bluejeans.com,*.bluejeans.com,*.a.bluejeans.com,*.bbcollab.com");
-#else
- // includes Mozilla's test domain: mozilla.github.io (not intended for release)
-pref("media.getusermedia.screensharing.allowed_domains", "mozilla.github.io,webex.com,*.webex.com,ciscospark.com,*.ciscospark.com,projectsquared.com,*.projectsquared.com,*.room.co,room.co,beta.talky.io,talky.io,*.clearslide.com,appear.in,*.appear.in,tokbox.com,*.tokbox.com,*.sso.francetelecom.fr,*.si.francetelecom.fr,*.sso.infra.ftgroup,*.multimedia-conference.orange-business.com,*.espacecollaboration.orange-business.com,free.gotomeeting.com,g2m.me,*.g2m.me,*.mypurecloud.com,*.mypurecloud.com.au,spreed.me,*.spreed.me,*.spreed.com,air.mozilla.org,*.circuit.com,*.yourcircuit.com,circuit.siemens.com,yourcircuit.siemens.com,circuitsandbox.net,*.unify.com,tandi.circuitsandbox.net,*.ericsson.net,*.cct.ericsson.net,*.opentok.com,*.conf.meetecho.com,meet.jit.si,*.meet.jit.si,web.stage.speakeasyapp.net,web.speakeasyapp.net,*.hipchat.me,*.beta-wspbx.com,*.wspbx.com,*.unifiedcloudit.com,*.smartboxuc.com,*.smartbox-uc.com,*.panterranetworks.com,pexipdemo.com,*.pexipdemo.com,pex.me,*.pex.me,*.rd.pexip.com,1click.io,*.1click.io,*.fuze.com,*.fuzemeeting.com,*.thinkingphones.com,gotomeeting.com,*.gotomeeting.com,gotowebinar.com,*.gotowebinar.com,gototraining.com,*.gototraining.com,citrix.com,*.citrix.com,expertcity.com,*.expertcity.com,citrixonline.com,*.citrixonline.com,g2m.me,*.g2m.me,gotomeet.me,*.gotomeet.me,gotomeet.at,*.gotomeet.at,miriadaxdes.miriadax.net,certificacion.miriadax.net,miriadax.net,*.wire.com,sylaps.com,*.sylaps.com,bluejeans.com,*.bluejeans.com,*.a.bluejeans.com,*.bbcollab.com");
-#endif
-
 pref("media.getusermedia.audiocapture.enabled", false);
 
 // TextTrack WebVTT Region extension support.