diff --git a/src/components/NcAppNavigation/NcAppNavigation.vue b/src/components/NcAppNavigation/NcAppNavigation.vue
index 6d21f364..15750922 100644
--- a/src/components/NcAppNavigation/NcAppNavigation.vue
+++ b/src/components/NcAppNavigation/NcAppNavigation.vue
@@ -339,7 +339,6 @@ export default {
 	background-color: var(--color-main-background-blur, var(--color-main-background));
 	-webkit-backdrop-filter: var(--filter-background-blur, none);
 	backdrop-filter: var(--filter-background-blur, none);
-	border-inline-end: 1px solid var(--color-border);
 
 	&--close {
 		margin-left: calc(-1 * min($navigation-width, var(--app-navigation-max-width)));
@@ -378,10 +377,18 @@ export default {
 	}
 }
 
+// Add extra border for high contrast mode
+[data-themes*="highcontrast"] {
+	.app-navigation {
+		border-inline-end: 1px solid var(--color-border);
+	}
+}
+
 // When on mobile, we make the navigation slide over the NcAppContent
 @media only screen and (max-width: $breakpoint-mobile) {
 	.app-navigation {
 		position: absolute;
+		border-inline-end: 1px solid var(--color-border);
 	}
 }
 
diff --git a/tests/component/snapshots/components/NcAppNavigationItem/visual.spec.ts-snapshots/has-primary-button-styling-on-active-entry-with-editing-true-1-chromium-linux.png b/tests/component/snapshots/components/NcAppNavigationItem/visual.spec.ts-snapshots/has-primary-button-styling-on-active-entry-with-editing-true-1-chromium-linux.png
index 55693d3d..ba69a7c3 100644
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diff --git a/tests/component/snapshots/components/NcAppNavigationItem/visual.spec.ts-snapshots/has-primary-button-styling-on-active-route-with-editing-true-1-chromium-linux.png b/tests/component/snapshots/components/NcAppNavigationItem/visual.spec.ts-snapshots/has-primary-button-styling-on-active-route-with-editing-true-1-chromium-linux.png
index bea5351e..36b7fab7 100644
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diff --git a/tests/component/snapshots/components/NcAppNavigationItem/visual.spec.ts-snapshots/has-primary-styling-on-active-entry-1-chromium-linux.png b/tests/component/snapshots/components/NcAppNavigationItem/visual.spec.ts-snapshots/has-primary-styling-on-active-entry-1-chromium-linux.png
index 1d2720a1..dc48ddf9 100644
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diff --git a/tests/component/snapshots/components/NcAppNavigationItem/visual.spec.ts-snapshots/has-primary-styling-on-active-route-1-chromium-linux.png b/tests/component/snapshots/components/NcAppNavigationItem/visual.spec.ts-snapshots/has-primary-styling-on-active-route-1-chromium-linux.png
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diff --git a/tests/component/snapshots/components/NcAppNavigationItem/visual.spec.ts-snapshots/has-tertiary-styling-on-non-active-entry-1-chromium-linux.png b/tests/component/snapshots/components/NcAppNavigationItem/visual.spec.ts-snapshots/has-tertiary-styling-on-non-active-entry-1-chromium-linux.png
index a5b4c77c..5e83bdca 100644
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diff --git a/tests/component/snapshots/components/NcAppNavigationItem/visual.spec.ts-snapshots/has-tertiary-styling-on-non-active-route-1-chromium-linux.png b/tests/component/snapshots/components/NcAppNavigationItem/visual.spec.ts-snapshots/has-tertiary-styling-on-non-active-route-1-chromium-linux.png
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